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The novel murine B cell differentiation antigen Lp-3
1Department of Pathology, Juntendo University School of Medicine, Tokyo, Japan.
International Immunology
|January 1, 1989
Summary
Researchers identified Lp-3, a novel B cell activation antigen, useful for distinguishing B cell differentiation stages. This antigen
Area of Science:
- Immunology
- Cell Biology
- Hematology
Background:
- The Lp-3 antigen is a unique murine lymphocyte differentiation antigen.
- It has a molecular weight of approximately 125 kd and was identified using a rat monoclonal antibody.
- Lp-3 is expressed on various myeloid, T cell, and B cell lineages in mice.
Purpose of the Study:
- To characterize the expression patterns of the Lp-3 antigen during B cell differentiation and activation.
- To determine the utility of Lp-3 as a marker for specific B cell populations.
- To investigate Lp-3 expression in autoimmune-prone mice.
Main Methods:
- Monoclonal antibody production and characterization.
- Flow cytometry for antigen expression analysis on various B cell subsets.
- Cell cycle analysis to determine Lp-3 expression timing during activation.
- Comparison of Lp-3 expression in BALB/c and NZB x NZW F1 mice.
Main Results:
- Lp-3 expression is stage-specific in B cells, found weakly on pre-B and virgin B cells, and absent on resting splenic B cells.
- Peritoneal cavity B cells, particularly Ly-1 (CD5) B cells, show brighter Lp-3 fluorescence.
- Lp-3 is induced on lipopolysaccharide-stimulated B cells and expressed early in the G1A phase of the cell cycle.
- Elevated Lp-3 expression is observed in the spleen of autoimmune-prone NZB x NZW F1 mice.
Conclusions:
- Lp-3 is identified as an early B cell activation antigen.
- Lp-3 is a valuable marker for distinguishing pre-B, virgin B, resting B, and activated B cell populations.
- Altered Lp-3 expression in autoimmune-prone mice suggests a potential role in autoimmune pathogenesis.