Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Collagen fibril surface structures: freeze-etching data and computer modelling.

M Raspanti1, F Ortolani, A Ruggeri

  • 1Istituto di Anatomia Umana Normale, Bologna, Italy.

International Journal of Biological Macromolecules
|April 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early multimodal behavioral cues in autism: a micro-analytical exploration of actions, gestures and speech during naturalistic parent-child interactions.

International journal of clinical and health psychology : IJCHP·2026
Same author

Indications for haematopoietic cell transplantation and CAR-T for haematological diseases, solid tumours and immune disorders: 2025 EBMT practice recommendations.

Bone marrow transplantation·2025
Same author

CC486 as a safe and effective bridge to transplant in MDS-del5q with transfusion-dependent anemia following lenalidomide relapse: A case report.

Current research in translational medicine·2025
Same author

The use of MSCs in steroid-refractory acute GvHD in Europe: a survey from the EBMT cellular therapy & immunobiology working party.

Bone marrow transplantation·2025
Same author

High-dose chemotherapy with autologous stem cell transplants in adult primary non-seminoma mediastinal germ-cell tumors. A report from the Cellular Therapy and Immunobiology working party of the EBMT.

ESMO open·2024
Same author

Refinement and revalidation of the Equine Ophthalmic Pain Scale: R-EOPS a new scale for ocular pain assessment in horses.

Veterinary journal (London, England : 1997)·2024
Same journal

Marek's disease virus-encoded microRNA-M6-5p suppresses viral replication by targeting UL42.

International journal of biological macromolecules·2026
Same journal

Pioneering the formation of 2-carboxylic anthraquinone: CRISPR/Cas9-mediated functional validation of Octaketide synthase and Polyketide reductase genes in Aloe vera.

International journal of biological macromolecules·2026
Same journal

Characterization of the blueberry AUXIN RESISTANT 1/LIKE-AUX1 gene family and regulatory roles of VcAUX1/LAX12 in flower bud burst.

International journal of biological macromolecules·2026
Same journal

Structural and biochemical insights into an anthocyanin-related glutathione transferase from bilberry and its inhibition by quercetin.

International journal of biological macromolecules·2026
Same journal

Hydrolases enzymes for oral biofilm reduction: Lipase, lysozyme, and amylase as promising candidates for canine oral health applications.

International journal of biological macromolecules·2026
Same journal

Dietary chitosan alleviates Enterohemorrhagic Escherichia coli induced intestinal injury by reducing bacterial colonization and restoring mucosal immune homeostasis in weaned piglets.

International journal of biological macromolecules·2026
See all related articles

Collagen fibril models closely match freeze-etched structures, revealing key features like the gap-overlap ratio. Minor ridges may result from collagen modifications or adhering materials, not primary structure.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Biomaterials Science

Background:

  • Collagen is a crucial structural protein.
  • Understanding collagen fibril structure is vital for tissue engineering and disease research.
  • The relationship between collagen's primary amino acid sequence and its fibril surface topography remains incompletely understood.

Purpose of the Study:

  • To investigate the correlation between the primary amino acid sequence of collagen and its observed surface profile.
  • To compare a computational model of collagen fibrils with experimentally visualized fibrils.
  • To identify structural features of collagen fibrils that may arise from post-translational modifications or external factors.

Main Methods:

  • Generation of a collagen fibril model directly from its amino acid sequence.

Related Experiment Videos

  • Comparative analysis of the fibril model with freeze-fractured and deep-etched collagen fibrils.
  • Detailed examination of surface features including gap-overlap ratio and intraperiod ridges.

    • Main Results:

    • The collagen fibril model closely replicated key features of experimentally observed fibrils, including the gap-overlap ratio and major ridges (X3, X2).
    • The model did not reproduce minor ridges (X1, Y1) observed in the gap zones of experimental samples.
    • This discrepancy suggests that minor ridges may not solely derive from the primary collagen structure.

    Conclusions:

    • The primary amino acid sequence of collagen largely dictates the main structural features of its fibrils.
    • Minor surface ridges (X1, Y1) in collagen fibrils may originate from post-translational modifications of collagen.
    • Alternatively, minor ridges could be attributed to non-collagenic materials that adhere to the fibril surface.