Related Experiment Video
Updated: Apr 28, 2026

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
Published on: January 12, 2019
Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice
Madoka Ayano1, Takahiro Kani, Mikiko Kojima
1Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi, 464-8601.
Abstract:
Under flooded conditions, the leaves and internodes of deepwater rice can elongate above the water surface to capture oxygen and prevent drowning. Our previous studies showed that three major quantitative trait loci (QTL) regulate deepwater-dependent internode elongation in deepwater rice. In this study, we investigated the age-dependent internode elongation in deepwater rice. We also investigated the relationship between deepwater-dependent internode elongation and the phytohormone gibberellin (GA) by physiological and genetic approach using a QTL pyramiding line (NIL-1 + 3 + 12). Deepwater rice did not show internode elongation before the sixth leaf stage under deepwater condition. Additionally, deepwater-dependent internode elongation occurred on the sixth and seventh internodes during the sixth leaf stage. These results indicate that deepwater rice could not start internode elongation until the sixth leaf stage. Ultra-performance liquid chromatography tandem mass-spectrometry (UPLC-MS/MS) method for the phytohormone contents showed a deepwater-dependent GA1 and GA4 accumulation in deepwater rice. Additionally, a GA inhibitor abolished deepwater-dependent internode elongation in deepwater rice. On the contrary, GA feeding mimicked internode elongation under ordinary growth conditions. However, mutations in GA biosynthesis and signal transduction genes blocked deepwater-dependent internode elongation. These data suggested that GA biosynthesis and signal transduction are essential for deepwater-dependent internode elongation in deepwater rice.
Related Concept Videos
Responses to Drought and Flooding
Cell Signaling in Plants
Gene Regulation During Sporulation
Short-distance Transport of Resources
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Stringent Response in E. coli

