Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Case Report of Anaplastic Lymphoma Kinase-Negative Inflammatory Myofibroblastic Tumor in the Anterior Mediastinum of an Elderly Male.

Journal of visualized experiments : JoVE·2026
Same author

Peri-head distance coding in the mouse brainstem.

Neuron·2026
Same author

Zinc Inhibits BTK Phosphorylation in Macrophages to Ameliorate Encephalitis in Neurotropic Virus-Infected Mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Integrated Protocol for Assessing Meningeal Lymphatics: Cisterna Magna Injection, Lymph Node Imaging, Meningeal Dissection, and Whole-Mount Staining.

Journal of visualized experiments : JoVE·2026
Same author

RNA-programmable cell-type monitoring and manipulation in the human cortex with CellREADR.

Cell reports·2026
Same author

Acute myeloid leukemia with mast cell differentiation evolving into mast cell leukemia.

Cytometry. Part B, Clinical cytometry·2026

Related Experiment Video

Updated: Apr 28, 2026

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

7.5K

SUMO2 is essential while SUMO3 is dispensable for mouse embryonic development.

Liangli Wang1, Carolien Wansleeben2, Shengli Zhao3

  • 1Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology, Duke University Medical Center, Durham, NC, USA.

EMBO Reports
|June 4, 2014
PubMed
Summary

Small ubiquitin-like modifier (SUMO) conjugation is vital for embryonic development. SUMO2 deficiency causes severe developmental delay and embryonic lethality, indicating SUMO2 expression levels are critical for embryogenesis.

Keywords:
Embryonic developmentKnockoutSUMO conjugationSUMO2SUMO3

More Related Videos

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

6.7K
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

8.1K

Related Experiment Videos

Last Updated: Apr 28, 2026

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

7.5K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

6.7K
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

8.1K

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Small ubiquitin-like modifier (SUMO) conjugation is essential for embryogenesis.
  • SUMO-conjugating enzyme Ubc9 deficiency leads to early embryonic lethality.
  • SUMO1 knockout mice are viable due to SUMO2/3 functional compensation.

Purpose of the Study:

  • To investigate the specific roles of SUMO2 and SUMO3 in mouse embryogenesis.
  • To determine if functional differences or expression levels of SUMO2/3 are critical for development.

Main Methods:

  • Generation of Sumo2- and Sumo3-null mutant mice.
  • Phenotypic analysis of knockout mice at various embryonic and postnatal stages.
  • Genotyping and comparative analysis of littermates.

Main Results:

  • Sumo3(-/-) mice were viable, but Sumo2(-/-) embryos showed severe developmental delay and died around embryonic day 10.5.
  • SUMO2 was identified as the predominantly expressed SUMO isoform during embryogenesis.
  • Compound heterozygotes (Sumo2(+/-);Sumo3(+/-)) were normal, but Sumo2(+/-);Sumo3(-/-) mice were rare at birth and significantly smaller, indicating a gene dosage effect.

Conclusions:

  • SUMO2 plays a critical, non-redundant role in embryogenesis, likely due to its predominant expression.
  • The findings suggest that expression levels, rather than inherent functional differences between SUMO2 and SUMO3, are crucial for normal embryonic development.