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Updated: Apr 28, 2026

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
Published on: December 10, 2013
Hesperidin inhibits inflammatory response induced by Aeromonas hydrophila infection and alters CD4+/CD8+ T cell ratio
Abdelaziz S A Abuelsaad1, Gamal Allam1, Adnan A A Al-Solumani2
1Department of Microbiology (Immunology Section), College of Medicine, Taif University, Taif 21974, Saudi Arabia ; Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Background:
Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of human diseases. Hesperidin (HES) has been reported to exert antioxidant and anti-inflammatory activities.
Objectives:
The aim of this study was to investigate the potential effect of HES treatment on inflammatory response induced by A. hydrophila infection in murine.
Methods:
A. hydrophila-infected mice were treated with HES at 250 mg/kg b.wt./week for 4 consecutive weeks. Phagocytosis, reactive oxygen species production, CD4(+)/CD8(+) T cell ratio, and CD14 expression on intestinal infiltrating monocytes were evaluated. The expression of E-selectin and intercellular adhesion molecule 1 on stimulated HUVECs and RAW macrophage was evaluated.
Results:
Percentage of CD4(+) T cells in the intestinal tissues of infected treated mice was highly significantly increased; however, phagocytic index, ROS production, CD8(+) T cells percentage, and CD14 expression on monocytes were significantly reduced. On the other hand, HES significantly inhibited A-LPS- and A-ECP-induced E-selectin and ICAM-1 expression on HUVECs and ICAM-1 expression on RAW macrophage.
Conclusion:
Present data indicated that HES has a potential role in the suppression of inflammatory response induced by A. hydrophila toxins through downmodulation of ROS production and CD14 and adhesion molecules expression, as well as increase of CD4(+)/CD8(+) cell ratio.
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