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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Fluorescent probe-based lateral flow assay for multiplex nucleic acid detection.

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Biosensor Technology

Background:

  • Multiplex detection of human papillomavirus (HPV) is crucial for cervical cancer screening.
  • Existing methods can be time-consuming, costly, or lack multiplexing capabilities.

Purpose of the Study:

  • To develop a rapid, low-cost, disposable dipstick-type DNA biosensor for simultaneous multiplex HPV detection.
  • To enable single-step, high-throughput HPV typing using a portable fluorescence reader.

Main Methods:

  • Development of a dual-color fluorescent lateral flow nucleic acid testing system.
  • Integration of fluorescently labeled probes with immobilized probes in an array format.
  • Utilizing linear-after-the-exponential (LATE)-PCR for DNA amplification.

Main Results:

  • Simultaneous detection of up to 13 HPV types in less than 30 minutes.
  • Achieved a sensitivity of 10-10(2) copies plasmid DNA/μL.
  • Demonstrated high specificity with no cross-reactivity among 31 common HPV types.
  • Clinical validation showed 95.3% overall agreement with a commercial kit.

Conclusions:

  • The developed DNA biosensor offers a rapid, cost-effective, and sensitive solution for multiplex HPV detection.
  • The technology shows significant potential for widespread clinical application in HPV diagnostics.