Related Experiment Video
Updated: Apr 28, 2026
Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
Published on: January 27, 2019
[Concordance of two serological tests for the diagnosis of Chagas disease]
Ana E Farfán-García1, Yeny Z Castellanos-Domínguez1, Katherine P Luna-Marín1
1Centro de Investigaciones en Enfermedades Tropicales -CINTROP-, Universidad Industrial de Santander, Colombia.
Objective:
Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.
Methodology:
A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.
Results:
The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.
Conclusions:
The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.
