Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Familial clustering of intrahepatic cholestasis of pregnancy: A nationwide population-based study in Denmark.

Hepatology (Baltimore, Md.)·2023

Same author

Upregulated estrogen receptors impairs myogenesis and elevates adipogenesis related factor levels in the paravertebral muscles of patients with idiopathic scoliosis.

Biochemical and biophysical research communications·2023

Same author

Evaluation of Maternal Infection During Pregnancy and Childhood Leukemia Among Offspring in Denmark.

JAMA network open·2023

Same author

Non-covalent ligand-oxide interaction promotes oxygen evolution.

Nature communications·2023

Same author

High Efficiency Near-Infrared Perovskite Light Emitting Diodes With Reduced Rolling-Off by Surface Post-Treatment.

Small (Weinheim an der Bergstrasse, Germany)·2023

Same author

Maternal hypertensive disorders during pregnancy and the risk of offspring diabetes mellitus in childhood, adolescence, and early adulthood: a nationwide population-based cohort study.

BMC medicine·2023

Search research articles

Related Experiment Video

Updated: Apr 28, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

Rapid and multiplex microRNA detection on graphically encoded silica suspension array.

Li Jiang¹, Ye Shen¹, Kexiao Zheng¹

¹Key Laboratory for Nano-Bio Interface Research, Nano-Bio-Chem Centre, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.

Biosensors & Bioelectronics

|June 4, 2014

Summary

This study introduces a novel fluorescence strategy for detecting microRNAs (miRNAs) using a silica suspension array. The method offers sensitive and specific detection of cancer biomarkers, enabling precise diagnosis.

Keywords:

Base stacking Graphical encoding MicroRNA Suspension array

More Related Videos

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Related Experiment Videos

Last Updated: Apr 28, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

MicroRNAs (miRNAs) are crucial noncoding RNA molecules recognized as promising biomarkers for cancer diagnosis.
Existing detection methods for miRNAs face challenges in sensitivity and specificity.
Suspension array technologies offer potential for multiplexed biomarker analysis.

Purpose of the Study:

To develop a label-free, multiplex miRNA detection strategy using a graphically encoded silica suspension array.
To leverage hybridization-triggered fluorescence and base-stacking effects for enhanced miRNA detection.
To assess the method's specificity, sensitivity, and potential for clinical applications.

Main Methods:

A hybridization-triggered fluorescence strategy was employed on a silica suspension array.
An 8-mer Universal Tag facilitated direct RNA capture via base-stacking interactions with capture probes.
The method was evaluated for its ability to discriminate single-nucleotide variations and distinguish mature miRNAs from pre-miRNAs.

Main Results:

The novel method demonstrated superb discrimination of single-nucleotide alterations at both 5' and 3' ends of miRNAs.
Distinguishing mature miRNAs from their precursor forms was achieved with high accuracy.
The detection limit was estimated at 5 amol, comparable to highly sensitive existing methods.
Multiplex detection of miRNAs was successfully demonstrated on the suspension array.

Conclusions:

The developed strategy provides a sensitive and specific platform for label-free, multiplex miRNA detection.
The method's ability to discern sequence variations holds significant potential for cancer biomarker discovery and clinical diagnostics.
This approach offers a promising tool for advancing miRNA-based diagnostics and research.