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Assessing acceptor substrate promiscuity of YjiC-mediated glycosylation toward flavonoids
Ramesh Prasad Pandey1, Rit Bahadur Gurung1, Prakash Parajuli1
1Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
Abstract:
The acceptor substrate promiscuity of YjiC, a UDP-glycosyltransferase from Bacillus licheniformis, was explored with seven different classes (flavonols, flavanols, flavones, flavanones, chalcone, stilbene, and isoflavonoids) of 23 flavonoid acceptors. For most of the polyphenols used in the reactions, the enzymatic bioconversion was significantly higher with the production of multiple glucosylated derivatives. This study highlights the highly flexible non-regiospecific glycosylation ability of YjiC toward polyphenolic compounds. The catalytic potential of YjiC could be useful to generate a library of natural product glucosides.
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