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Related Experiment Video

Updated: Apr 28, 2026

The FlyBar: Administering Alcohol to Flies

The FlyBar: Administering Alcohol to Flies

Published on: May 18, 2014

The FlyBar: administering alcohol to flies.

Kim van der Linde¹, Emiliano Fumagalli², Gregg Roman²

¹Department of Biological Science, Florida State University.

Journal of Visualized Experiments : Jove

|June 5, 2014

Summary

This study details a protocol for measuring alcohol sensitivity in fruit flies (Drosophila melanogaster) using controlled alcohol vapor exposure. The method allows for precise analysis of how the circadian clock influences alcohol

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Last Updated: Apr 28, 2026

The FlyBar: Administering Alcohol to Flies

The FlyBar: Administering Alcohol to Flies

Published on: May 18, 2014

High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster

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Published on: April 20, 2020

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

Published on: April 15, 2015

Area of Science:

Circadian Biology
Neuroscience
Pharmacology

Background:

Fruit flies (Drosophila melanogaster) are a key model organism for studying alcohol effects and circadian rhythms.
Previous research indicated the circadian clock influences alcohol sensitivity but not tolerance.
A detailed protocol is needed to standardize experiments in this area.

Purpose of the Study:

To describe a detailed protocol for assessing alcohol sensitivity in Drosophila melanogaster.
To enable precise measurement of alcohol-induced responses in flies.
To facilitate research on the interaction between circadian rhythms and alcohol sensitivity.

Main Methods:

Flies are exposed to controlled alcohol vapor concentrations using the FlyBar system.
Standardized rearing conditions and age-matched flies (3-day old) are used.
Loss of Righting Reflex (LoRR) is recorded every 5 minutes over a 1-hour exposure period.

Main Results:

The protocol allows for quantitative measurement of alcohol-induced sedation (LoRR).
Data analysis includes determining median LoRR time, percentage LoRR at specific times, or using multivariate time-series analysis.
The method is adaptable for comparing different genotypes or experimental conditions.

Conclusions:

This protocol provides a robust and reproducible method for studying alcohol sensitivity in Drosophila.
It allows for detailed investigation into the role of the circadian clock in modulating alcohol responses.
The standardized approach supports comparative studies in chronobiology and alcohol research.