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Published on: January 14, 2014
IDH1(R132H) mutation increases U87 glioma cell sensitivity to radiation therapy in hypoxia
Xiao-Wei Wang1, Marianne Labussière1, Samuel Valable2
1Université Pierre et Marie Curie-Paris 6, Centre de Recherche de l'Institut du Cerveau et de la Moëlle épinière (CRICM), UMR-S975, 75013 Paris, France ; INSERM, U 975, 75013 Paris, France ; CNRS, UMR 7225, 75013 Paris, France.
Objective:
IDH1 codon 132 mutation (mostly Arg132His) is frequently found in gliomas and is associated with longer survival. However, it is still unclear whether IDH1 mutation renders the cell more vulnerable to current treatment, radio- and chemotherapy.
Materials And Methods:
We transduced U87 with wild type IDH1 or IDH1 (R132H) expressing lentivirus and analyzed the radiosensitivity (dose ranging 0 to 10 Gy) under normoxia (20% O2) and moderate hypoxia (1% O2).
Results:
We observed that IDH1 (R132H) U87 cells grow faster in hypoxia and were more sensitive to radiotherapy (in terms of cell mortality and colony formation assay) compared to nontransduced U87 and IDH1 (wt) cells. This effect was not observed in normoxia.
Conclusion:
These data suggest that IDH1 (R132H) mutation increases radiosensitivity in mild hypoxic conditions.
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