Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Tissue Archives to Liquid Biopsy: Transfer Learning for MicroRNA-Based Lung Cancer Diagnosis.

Analytical chemistry·2026
Same author

Effects of Aptamer-Functionalized Multivalent DNA Nanostructures on Small-Molecule Detection in Wearable Sweat Sensors.

ACS sensors·2025
Same author

Programmable DNA Nanospheres for Photothermal-Controlled Intracellular Protein Degradation.

Angewandte Chemie (International ed. in English)·2025
Same author

Entropy-Driven Disassembly of Logic-Gated DNA Nanospheres: An All-in-One Platform for Precise Cancer Diagnosis In Vivo.

ACS nano·2025
Same author

Digital Microfluidic Platform Based on Printed Circuit Board for Affinity Evaluation of Mertansine Aptamers.

Analytical chemistry·2025
Same author

Efficacy of unilateral biportal endoscopy vs. unilateral portal endoscopy for the treatment of lumbar spinal stenosis: a systematic review and meta-analysis.

Frontiers in surgery·2025

Related Experiment Video

Updated: Apr 28, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

7.6K

Nucleic acid aptamers for living cell analysis.

Xiangling Xiong1, Yifan Lv, Tao Chen

  • 1Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Molecular Engineering and Theranostics, Hunan University, Changsha 410082, China.

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|June 5, 2014
PubMed
Summary

Nucleic acid aptamers, acting as "chemical antibodies," offer powerful solutions for analyzing cell structures and functions. This review highlights their advances in cell membrane analysis, detection, isolation, and intracellular studies.

Keywords:
aptamercell detection and isolationcell membrane analysisreal-time monitoring

More Related Videos

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

4.1K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.3K

Related Experiment Videos

Last Updated: Apr 28, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

7.6K
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

4.1K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.3K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Cells are fundamental units of life, and understanding their structure is key to elucidating biological functions.
  • Distinguishing between healthy and diseased cells is crucial for understanding pathology, diagnostics, and therapeutics.
  • Monitoring living cells presents significant challenges in bioanalytical and life science research.

Purpose of the Study:

  • To review recent advancements in nucleic acid aptamer development for cellular analysis.
  • To explore the application of aptamers in various aspects of cell biology research.
  • To discuss the limitations and potential solutions for aptamer-based cell studies.

Main Methods:

  • Review of recent literature on nucleic acid aptamer development and applications.
  • Focus on aptamers used in cell membrane analysis, detection, isolation, and intracellular studies.
  • Discussion of aptamer utility in real-time monitoring of cell secretion and intracellular delivery.

Main Results:

  • Aptamers have emerged as effective tools for cellular analysis, functioning as
  • chemical antibodies.
  • Significant progress has been made in applying aptamers to cell membrane analysis, cell detection and isolation.
  • Aptamers show promise for real-time monitoring of cell secretion and intracellular delivery/analysis in living cell models.

Conclusions:

  • Nucleic acid aptamers are increasingly valuable tools for overcoming challenges in living cell monitoring.
  • Further development and application of aptamers can significantly advance bioanalytical and life science research.
  • Addressing current limitations will enhance the utility of aptamers in diverse cellular studies.