Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Getting pumped about heart failure.

Douglas L Mann1

  • 1Center for Cardiovascular Research, Division of Cardiology, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.

Cell Metabolism
|June 5, 2014
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

What E.B. Meant to Me.

Journal of the American College of Cardiology·2026
Same author

Vagal Nerve Stimulation in Patients With Heart Failure and Reduced Ejection Fraction: The ANTHEM-HFrEF Trial.

Journal of the American College of Cardiology·2026
Same author

In Defense of Homeostasis: Innate Immunity in Cardiac Injury and Repair.

Immunological reviews·2026
Same author

Body Mass Index, Clinical Outcomes, and Mortality in Heart Failure: A Mendelian Randomization Study.

Journal of the American College of Cardiology·2026
Same author

RACGAP1 as a Circulating Biomarker of Atrial Fibrillation in Heart Failure: A Dual-Cohort Proteomic Study.

Circulation. Arrhythmia and electrophysiology·2026
Same author

Targeting Inflammation in Heart Failure: Déjà Vu All Over Again or a Therapeutic Step Forward?

JACC. Heart failure·2026
Same journal

AARS1 promotes tumor progression and immune evasion via ATF6 lactylation-mediated tryptophan metabolism in hepatocellular carcinoma.

Cell metabolism·2026
Same journal

Reactive species as regulators of immune cell metabolism, tolerance, and autoimmunity.

Cell metabolism·2026
Same journal

The interplay between the microbiome and immune cells in metabolic homeostasis and disease.

Cell metabolism·2026
Same journal

The metabolic basis of regulated cell death.

Cell metabolism·2026
Same journal

Gut microbiota-derived lysine phenylacetylation impairs mitochondrial function and is alleviated by SIRT3.

Cell metabolism·2026
Same journal

Methionine-supplemented longevity diet increases growth hormone, GLP-1, and FGF21; reduces frailty; and promotes healthspan.

Cell metabolism·2026
See all related articles

Targeting microRNA-25 with antisense oligonucleotides reversed heart failure pump dysfunction in mice. This approach improved cardiac function and survival by restoring proper calcium handling in heart muscle cells.

Area of Science:

  • Cardiovascular Science
  • Molecular Biology
  • Genetics

Background:

  • Heart failure is a syndrome characterized by impaired cardiac pumping capacity.
  • Dysregulated calcium handling within cardiomyocytes is a key mechanism underlying heart failure.
  • MicroRNAs play critical roles in regulating cardiac function.

Purpose of the Study:

  • To investigate the role of microRNA-25 in cardiac calcium handling and pump function.
  • To determine if targeting microRNA-25 can ameliorate heart failure phenotypes in a mouse model.

Main Methods:

  • Utilized a mouse model of heart failure.
  • Assessed cardiac calcium handling and contractility.
  • Administered antisense oligonucleotides targeting microRNA-25.

Related Experiment Videos

Main Results:

  • Upregulation of microRNA-25 was found to impair cardiac calcium handling and lead to pump dysfunction.
  • Antisense oligonucleotide treatment targeting microRNA-25 reversed cardiac pump dysfunction.
  • Targeting microRNA-25 improved survival rates in mice with heart failure.

Conclusions:

  • MicroRNA-25 is a critical regulator of cardiac calcium handling and pump function.
  • Antisense oligonucleotide-mediated inhibition of microRNA-25 represents a potential therapeutic strategy for heart failure.
  • Restoring normal calcium handling through microRNA targeting offers a promising avenue for improving heart failure outcomes.