Related Experiment Video

Updated: Apr 28, 2026

Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
08:24

Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins

Published on: November 18, 2016

10.9K

Characterization of SET/I2PP2A isoforms in dogs

Ryotaro Yabe1, Nobuyuki Fujiwara, Takuya Mizuno

  • 1Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

The Journal of Veterinary Medical Science
|June 6, 2014
PubMed

Insights

Researchers identified four unique SET protein isoforms (SETα, β, γ, δ) in canine cells, crucial for developing targeted therapies for canine lymphoma. These isoforms differ in gene location and cellular localization, impacting their interaction with protein phosphatase 2A (PP2A).

Area of Science:

  • Veterinary Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • The SET protein, an endogenous inhibitor of protein phosphatase 2A (PP2A), is linked to poor prognosis in human leukemia.
  • Previous research indicated elevated SET protein levels in canine lymphoma cell lines, suggesting potential therapeutic strategies using SET antagonists.

Purpose of the Study:

  • To investigate the diversity of SET protein isoforms expressed in canine cells.
  • To characterize the genetic location, cellular localization, and PP2A-binding capabilities of these canine SET isoforms.

Main Methods:

  • Cloning of four SET isoforms: SETα, SETβ, SETγ, and SETδ.
  • Genomic BLAST analysis to determine gene locations on canine chromosomes.
  • Immunofluorescence studies to ascertain subcellular localization.
  • Confirmation of SETα and SETβ association with PP2A.

Main Results:

  • Four distinct SET isoforms (SETα, β, γ, δ) were identified and cloned from canine cells.
  • SET genes were mapped to chromosomes X, 7, 1, and 8, respectively.
  • SETα and SETβ exhibited nuclear localization, while SETγ and SETδ showed both nuclear and cytosolic distribution.
  • SETα and SETβ were confirmed to bind with PP2A.

Conclusions:

  • Canine cells express unique SET protein isoforms with varying characteristics.
  • These distinct isoforms necessitate consideration in the development of SET-targeting drugs for canine lymphoma.
  • Understanding these isoforms is critical for advancing therapeutic strategies in veterinary oncology.

More Related Videos

Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia09:36

Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

Published on: December 22, 2023

2.0K
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification08:55

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Published on: November 20, 2017

11.7K

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
7.6K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.2K
Cushing Syndrome II: Pathophysiology01:19

Cushing Syndrome II: Pathophysiology

Cortisol production is normally governed by the hypothalamic–pituitary–adrenal (HPA) axis, which maintains hormonal balance through tightly regulated feedback mechanisms. Disruption of this regulatory system is central to the development of Cushing syndrome, whether the excess cortisol originates from external medications or internal pathology. Persistent cortisol elevation alters metabolism, immune function, and endocrine signaling, producing the characteristic clinical features...
24
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
1.2K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.8K
Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.0K