[Influence of sucrose on aspirin ulcer]

At the present stage of drug interactions, little has been studied on food-drug interactions. Excess intake of sucrose is well known to cause arteriosclerosis, obesity, diabetes mellitus, toxicity to blood cells, dental caries, and accumulations of metabolic products in blood. Under the such conditions, it is possible that the ulcerogenic drugs, such as synthetic anti-inflammatory drugs, may cause severe gastric lesions by oral administration. The present investigation was undertaken to study the influences of sucrose on the gastric lesions induced by aspirin (aspirin ulcer) in rats maintained with 10% sucrose solution. Thus, the effect of sucrose on aggressive and protective factors, proposed by Shay and Sun, was studied.

Sixty rats were maintained with 10% sucrose solution ad libitum as drinking solution for 4 weeks (10% sucrose group). As control, purified water was given ad libitum to 60 rats (control group). Rats were starved for 24 hours, but allowed free access of water before the experiment, and then were subjected to the following experiments: (1) Twenty rats from each group were treated to prepare pylorus-ligated rats. Aspirin in 1% CMC (100mg/kg body-weight) was given in 10 rats from each group immediately after the ligation. Gastric juice excreted during 6 hours was collected and its supernatant (1,500 x g) was used for determinations of pH, acidity, acid output, pepsin activity, and pepsin output. Aspirin and salicylic acid contents in gastric juice were also determined by HPLC. (2) The mucosa of the stomach of (1) were subjected to the analysis of hexosamine. Twenty rats from each group without pylorus-ligation were also used for the analysis. Among them, 10 rats from each group were given aspirin in 1% CMC with the dose of 300 mg/kg body-weight. (3) Ten rats from each group were given aspirin with the dose of 300 mg/kg body-weight and the stomach (n = 10 for each group) was extracted 30 minutes after the aspirin administration for histochemical study to obtain covering mucus index, epitherial mucus index, total mucus index, and rate of mucous injury. (4) Aspirin in 1% CMC was given to 10 rats of each group with the dose of 300 mg/kg body-weight. Rats were sacrificed 6 hours after the administration of aspirin, and then stomachs were extracted and treated with 10% neutral formalin. The length of lesions was measured under a microscope (x 10), and then Ulcer Index was obtained.(ABSTRACT TRUNCATED AT 400 WORDS)