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Blood Transfusion and Agglutination

Blood Transfusion and Agglutination

Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...

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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

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Defining a reference range for cold agglutinin titers.

Brenda J Bendix¹, Craig D Tauscher, Sandra C Bryant

¹Division of Transfusion Medicine, Mayo Clinic, Rochester, Minnesota.

|June 6, 2014

Summary

This summary is machine-generated.

Cold agglutinin (CAGG) titers in healthy donors are lower than in patients with cold agglutinin disease (CAD). This study established new reference ranges, finding no seasonal variation but a sex association in patients.

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Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Published on: January 29, 2010

Area of Science:

Hematology
Clinical Chemistry

Background:

Cold agglutinin (CAGG) titer testing is crucial for diagnosing cold agglutinin disease (CAD).
Existing reference ranges may not accurately reflect healthy populations.

Purpose of the Study:

To establish a seasonally adjusted reference range for CAGG titers using prospective samples from healthy blood donors.
To compare this new reference range with retrospectively generated ranges from patient cohorts.

Main Methods:

Prospective CAGG titer testing on healthy blood donors in January and July.
Retrospective analysis of patient CAGG titers using current and historical methods, excluding CAD-associated conditions.
Verification of results using reference range program volunteers.

Main Results:

No significant age or seasonal variation in CAGG titers was observed in blood donors or historical patient cohorts.
A significant sex association was found in the current patient cohort (males: 64-512, females: ≤64).
Prospective blood donor CAGG titers showed a lower 95% reference range (≤4) compared to historical (≤32) and current (≤64) patient cohorts.

Conclusions:

Prospective CAGG titers from healthy blood donors are lower than those from retrospective patient cohorts.
This difference may indicate that blood donors represent a healthier population, necessitating distinct reference ranges.