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Updated: Apr 28, 2026
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
Noninvasive and label-free determination of virus infected cells by Raman spectroscopy
Kamila Moor1, Kiyoshi Ohtani1, Diyas Myrzakozha2
1Kwansei Gakuin University, School of Science and Technology, Department of Bioscience, 2-1 Gakuen, Sanda, Hyogo 669-1337 Japan.
Abstract:
The present study demonstrates that Raman spectroscopy is a powerful tool for the detection of virus-infected cells. Adenovirus infection of human embryonic kidney 293 cells was successfully detected at 12, 24, and 48 h after initiating the infection. The score plot of principal component analysis discriminated the spectra of the infected cells from those of the control cells. The viral infection was confirmed by the conventional immunostaining method performed 24 h after the infection. The newly developed method provides a fast and label-free means for the detection of virus-infected cells.
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