Related Experiment Video
Updated: Apr 28, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
Surface analysis reveals biogenic oxidation of sub-bituminous coal by Pseudomonas fluorescens
Nur Hazlin Hazrin-Chong1, Christopher E Marjo, Theerthankar Das
1Centre for Marine Bioinnovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, NSW, 2052, Australia.
Abstract:
Direct analysis of the colonised surface on coal using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) revealed the nature of bacteria-mediated oxidation at the coal surface. Unique oxidation peaks generated by the presence of Pseudomonas fluorescens on coal was shown through ATR-FTIR measurements, and ATR-FTIR imaging illustrated that this peak was only observed within the region of coal colonised by bacteria. Contact angle measurements and surface free energy of adhesion calculations showed that the adhesion between P. fluorescens and coal was thermodynamically favourable, and scanning electron microscopy (SEM) exhibited individual cell or monolayer cluster attachment on coal. Furthermore, Gaussian peak fitting of peroxidase-treated coal ATR-FTIR spectra revealed that peroxidase or related enzymes produced by P. fluorescens may be responsible for coal oxidation. This study demonstrated the usefulness and practicality of ATR-FTIR for analysing coal oxidation by P. fluorescens and may well be applied to other microbe-driven modifications of coal for its rapidity and reliability.
More Related Videos
08:12Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids BPCA
Published on: May 16, 2016
13:11Using Flexible Gold-Titanium Reaction Cells to Simulate Pressure-Dependent Microbial Activity in the Context of Subsurface Biomining
Published on: October 5, 2019
Related Concept Videos
Bioremediation
Microbial Bioremediation of Hydrocarbons
Microbes and Methanogenesis
Anoxygenic Photosynthesis
Environmental Applications of Microorganisms
Anoxygenic Phototrophic Bacteria