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Gp130-dependent signaling in the podocyte
Yoshikuni Nagayama1, Gerald S Braun2, Christina M Jakobs3
1Division of Nephrology and Immunology, RWTH Aachen University, Aachen Germany; Division of Nephrology, Showa University Fujigaoka Hospital, Yokohama, Japan.
American Journal of Physiology. Renal Physiology
|June 6, 2014
Summary
Interleukin-6 (IL-6) signaling via gp130 in kidney podocytes is not essential for disease development in mouse models. Podocyte-specific gp130 deletion did not alter injury responses in LPS or nephrotoxic serum models.
Area of Science:
- Nephrology
- Immunology
- Molecular Biology
Background:
- Glomerular inflammation often involves elevated Interleukin-6 (IL-6) levels.
- The role of IL-6 signaling in glomerular podocytes, crucial for kidney function and disease, remains unclear in vivo.
Purpose of the Study:
- To investigate the in vivo relevance of IL-6/gp130 signaling in podocytes during kidney injury.
Main Methods:
- Generated mice with podocyte-specific deletion of gp130 (a key IL-6 family receptor subunit).
- Assessed podocyte IL-6 signaling via STAT3 phosphorylation.
- Utilized LPS and nephrotoxic serum models to induce kidney injury.
Main Results:
- Podocytes express gp130 and exhibit STAT3 phosphorylation upon IL-6 stimulation.
- Podocyte-specific gp130 deficient mice showed no spontaneous renal pathology.
- Deletion of podocyte gp130 did not alter disease severity in LPS-induced or crescentic nephritis models.
Conclusions:
- gp130-mediated IL-6 family signaling in podocytes is not critical for the development of major glomerular pathologies in the tested mouse models.
- Podocyte IL-6 signaling does not play a significant role in acute kidney injury responses investigated.
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