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Updated: Apr 28, 2026

Influenza A Virus Studies in a Mouse Model of Infection
Published on: September 7, 2017
Detection of influenza A antibodies in avian serum samples by ELISA
Len Chappell1, Mary Lea Killian, Erica Spackman
1Georgia Poultry Laboratory Network, Oak wood, GA, USA.
Abstract:
ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive, they provide a ready-to-use assay with good quality control. Various sample types can be processed for ELISA: serum, plasma, egg yolk, blood collected on filter paper. Quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, results interpretation, and troubleshooting are discussed.
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