Related Experiment Video
Updated: Apr 28, 2026
Nasal Wipes for Influenza A Virus Detection and Isolation from Swine
Published on: December 4, 2015
Antibody secreting cell assay for influenza A virus in swine
Pravina Kitikoon1, Crystal L Loving, Amy L Vincent
1Virus and Prion Research Unit, National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, 1920 Dayton Avenue, Ames, IA, 50010, USA.
An ELISPOT assay to enumerate B-cells producing antibodies specific to a given antigen, also known as an antibody secreting cell (ASC) assay, was adapted to detect B-cells specific for influenza A virus (IAV). The assay is performed ex vivo and enumerates ASC at a single cell level. A simple ASC detection method is based on a solid phase immunoenzymatic principle. The ELISPOT plate is coated with IAV prior to incubation with cell samples. The spot-forming cells are detected by enzyme labeled antibodies directed to the swine immunoglobulin (Ig) class of interest and visualized with the addition of substrate.
An ELISPOT assay to enumerate B-cells producing antibodies specific to a given antigen, also known as an antibody secreting cell (ASC) assay, was adapted to detect B-cells specific for influenza A virus (IAV). The assay is performed ex vivo and enumerates ASC at a single cell level. A simple ASC detection method is based on a solid phase immunoenzymatic principle. The ELISPOT plate is coated with IAV prior to incubation with cell samples. The spot-forming cells are detected by enzyme labeled antibodies directed to the swine immunoglobulin (Ig) class of interest and visualized with the addition of substrate.
