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Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
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Equine influenza culture methods.

Thomas M Chambers1, Stephanie E Reedy

  • 1Department of Veterinary Science, OIE Reference Laboratory for Equine Influenza, Maxwell H. Gluck Equine Research Center, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546-0099, USA, tmcham1@uky.edu.

Methods in Molecular Biology (Clifton, N.J.)
|June 6, 2014
PubMed
Summary
This summary is machine-generated.

Equine influenza viruses are cultured in eggs or MDCK cells. Equine-1 viruses kill embryos, while equine-2 viruses do not, indicating differences in host adaptation.

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Area of Science:

  • Veterinary Virology
  • Influenza Virus Research

Background:

  • Equine influenza viruses (EIVs) are significant pathogens affecting horse populations worldwide.
  • Standard methods for culturing influenza A viruses, including EIVs, involve embryonated hen eggs or mammalian cell lines like Madin-Darby canine kidney (MDCK) cells.
  • Host adaptation mutations can arise during virus propagation in these systems.

Purpose of the Study:

  • To investigate the replication and embryo-lethal effects of equine-1 (H7N7) and equine-2 (H3N8) influenza viruses in embryonated hen eggs.
  • To compare the behavior of different EIV subtypes during in ovo culture.

Main Methods:

  • Culturing of equine-1 (H7N7) and equine-2 (H3N8) influenza viruses in 11-day-old embryonated hen eggs.
  • Observation and recording of virus replication efficiency and effects on embryo viability.

Main Results:

  • Both equine-1 (H7N7) and equine-2 (H3N8) viruses replicated efficiently in 11-day-old embryonated hen eggs.
  • Equine-1 viruses were found to be lethal to the developing embryos.
  • Equine-2 viruses replicated without causing embryo mortality.

Conclusions:

  • Embryonated hen eggs are a suitable host for efficient replication of both equine-1 and equine-2 influenza viruses.
  • A significant difference exists in the pathogenicity of equine-1 and equine-2 viruses within embryonated eggs, with equine-1 exhibiting embryo lethality.
  • These findings highlight distinct host-pathogen interactions and potential differences in virulence or adaptation mechanisms between EIV subtypes.