Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antigenic structure of Coccidioides immitis.

R A Cox1

  • 1San Antonio State Chest Hospital, Texas.

Immunology Series
|January 1, 1989
PubMed
Summary

Coccidioides immitis fungal antigens, TP and CF, are key to host-parasite interactions in coccidioidomycosis. Understanding their composition and immunogenicity aids in disease management.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Atmospheric degradation of ozone depleting substances, their substitutes, and related species.

Chemical reviews·2015
Same author

Heterogeneous interaction of SiO2 with N2O5: aerosol flow tube and single particle optical levitation-Raman spectroscopy studies.

The journal of physical chemistry. A·2014
Same author

Optical trapping and Raman spectroscopy of solid particles.

Physical chemistry chemical physics : PCCP·2014
Same author

Evaluation of laboratory kinetics and photochemical data for atmospheric chemistry applications.

Chemical Society reviews·2012
Same author

Mechanism of atmospheric photooxidation of organic compounds. Reactions of alkoxy radicals in oxidation of n-butane and simple ketones.

Environmental science & technology·2012
Same author

Temperature dependent structured absorption spectra of molecular chlorine.

Physical chemistry chemical physics : PCCP·2011

Area of Science:

  • Mycology
  • Immunology
  • Infectious Diseases

Background:

  • Coccidioides immitis exhibits a challenging multiphasic life cycle, converting from arthroconidia to spherule-endospore phases in host tissues.
  • The fungal cell wall of C. immitis is a critical source of immunoreactive antigens involved in host-parasite interactions.
  • Previous studies have identified various antigens, including TP and CF, with differing compositions and immunogenic potentials.

Purpose of the Study:

  • To characterize the immunoreactive antigens from Coccidioides immitis cell walls.
  • To investigate the composition, molecular weight, and immunogenic properties of TP and CF antigens.
  • To explore the potential role of fungal antigens in the pathophysiology of coccidioidomycosis.

Main Methods:

  • Two-dimensional immunoblotting (2D-IEP) and Western blotting were employed for antigen analysis.
  • Compositional analyses were performed to determine the nature of the antigens (polysaccharide, glycolipoprotein, mannoprotein).
  • Chromatographic techniques (molecular sieve, ion-exchange, lectin affinity) were used to assess antigen association.

Main Results:

  • The TP antigen, predominantly polysaccharide (130-330 kD), may possess tolerogenic activity, inducing transient IgM responses.
  • The CF antigen, a heat-labile glycolipoprotein complex (43-48 kD), is highly immunogenic, inducing persistent IgG responses.
  • The skin test active component (Ag2) appears to be a mannoprotein, closely associated with the TP antigen.
  • Spherule extracts elicited stronger immune responses than mycelial extracts, possibly due to quantitative antigenic differences.

Conclusions:

  • Significant progress has been made in isolating and characterizing C. immitis antigens.
  • Further research is needed to fully understand antigen production, expression during morphogenetic phases, and their role in coccidioidomycosis pathophysiology.

Related Experiment Videos