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Updated: Apr 28, 2026
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Systemic approach to identify serum microRNAs as potential biomarkers for acute myocardial infarction
An Hsu1, Shu-Jen Chen2, Yu-Sun Chang3
1Graduate Institute of Biomedical Sciences, School of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan.
Background:
Recent studies have revealed the role of microRNAs (miRNAs) in a variety of biological and pathological processes, including acute myocardial infarction (AMI). We hypothesized that ST-segment elevation myocardial infarction (STEMI) may be associated with an alteration of miRNAs and that circulating miRNAs may be used as diagnostic markers for STEMI.
Methods:
Expression levels of 270 serum miRNAs were analyzed in 8 STEMI patients and 8 matched healthy controls to identify miRNAs differentially expressed in the sera of patients with AMI. The differentially expressed miRNAs were evaluated in a separate cohort of 62 subjects, including 31 STEMI patients and 31 normal controls.
Results:
The initial profiling study identified 12 upregulated and 13 downregulated serum miRNAs in the AMI samples. A subsequent validation study confirmed that serum miR-486-3p and miR-150-3p were upregulated while miR-126-3p, miR-26a-5p, and miR-191-5p were significantly downregulated in the sera of patients with AMI. Ratios between the level of upregulated and downregulated miRNAs were also significantly different in those with AMI. Receiver operator characteristics curve analysis using the expression ratio of miR-486-3p and miR-191-5p showed an area under the curve of 0.863.
Conclusion:
Our results suggest that serum miRNAs may be used as potential diagnostic biomarkers for STEMI.
Insights
Serum microRNAs (miRNAs) show altered levels in ST-segment elevation myocardial infarction (STEMI) patients. These circulating miRNAs, particularly miR-486-3p and miR-191-5p ratios, may serve as novel diagnostic biomarkers for STEMI.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiology
Background:
- MicroRNAs (miRNAs) play crucial roles in biological and pathological processes.
- Acute myocardial infarction (AMI) involves complex molecular changes.
- Alterations in circulating miRNAs are implicated in various diseases.
Purpose of the Study:
- To investigate alterations in serum miRNA expression in ST-segment elevation myocardial infarction (STEMI).
- To identify potential circulating miRNA biomarkers for STEMI diagnosis.
Main Methods:
- Serum miRNA expression profiling of 270 miRNAs in 8 STEMI patients and 8 healthy controls.
- Validation of differentially expressed miRNAs in a larger cohort of 31 STEMI patients and 31 controls.
- Receiver operator characteristics (ROC) curve analysis to assess diagnostic accuracy.
Main Results:
- 12 miRNAs were upregulated and 13 downregulated in STEMI patients' serum.
- miR-486-3p and miR-150-3p were significantly upregulated; miR-126-3p, miR-26a-5p, and miR-191-5p were downregulated.
- The ratio of miR-486-3p to miR-191-5p demonstrated a high diagnostic accuracy (AUC = 0.863).
Conclusions:
- Serum miRNA expression profiles are significantly altered in STEMI patients.
- Specific circulating miRNAs, such as miR-486-3p and miR-191-5p, show potential as diagnostic biomarkers for STEMI.
- Circulating miRNAs offer a promising non-invasive approach for STEMI diagnosis.
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