Related Experiment Video
Updated: Apr 28, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Oral health determinants and caries outcome among non-privileged children
Joana C Carvalho1, Eduardo F Silva, Elcio O Vieira
1Faculty of Medicine and Dentistry, Catholic University of Louvain, Brussels, Belgium.
Insights
Parental habits and nursery policies significantly impact early childhood caries. Interventions targeting bottle-feeding, toothbrushing assistance, and sugary snack intake in nurseries can improve children's oral health.
Area of Science:
- Pediatric Dentistry
- Public Health
- Epidemiology
Background:
- Early childhood caries (ECC) is a significant public health issue.
- Identifying modifiable determinants of ECC in non-privileged populations is crucial for targeted interventions.
- Understanding the influence of institutional environments, such as nurseries, on oral health is essential.
Purpose of the Study:
- To identify early childhood oral health determinants amenable to change.
- To investigate the impact of nursery policies on caries progression.
- To examine associations between parental practices, nursery policies, and caries in Brazilian children.
Main Methods:
- A multilevel model (generalized linear mixed model) was used.
- The study included 2,511 Brazilian children aged 1-5 years.
- Data collected via parent interviews and nursery questionnaires.
Main Results:
- Nighttime bottle-feeding, inadequate toothbrushing assistance, and infrequent dental visits were associated with higher caries rates.
- Nursery policies regarding sugary product intake (2-4 times daily) showed a significant association with caries.
- Maternal education level interacted with nursery policies and sugary intake, influencing caries risk.
Conclusions:
- Parental practices and nursery policies significantly influence children's oral health and caries development.
- Modifiable determinants, particularly those related to nursery policies, offer opportunities for intervention.
- Targeted oral health promotion in early childhood settings is recommended.
Abstract:
The study aimed at identifying oral health determinants that are present in early childhood, are amenable to change and for which there is evidence of their modulation of the rate of caries progression in a sample of non-privileged children. The null hypothesis was that determinants associated with the child's institutional environment as evidenced by nursery policies significantly influenced the child's oral health. The sample was formed by 2,511 Brazilian 1-5-year-olds. Caries severity and activity were recorded. Parents were interviewed and nurseries answered a questionnaire. According to the case status d1efs, the final multilevel model (generalized linear mixed model) identified significant associations with determinants such as bottle-feeding the child during the night on demand, not assisting the child in toothbrushing, ensuring visit to the dentist in case of troubles with teeth only (p < 0.0001) and intake of sugary products 2-4 times daily at nursery (p = 0.026). The likelihood of caries outcomes was associated with determinants related to nursery policies, not promoting preventive oral health care for children of mothers with 4 years of education (OR = 2.14; p < 0.0015) and <4 years of education (OR = 2.6; p = 0.0010), as well as consumption of sugary products 2-4 times daily for children of mothers with 4 years of education (OR = 3.35; p = 0.0010) and <4 years of education (OR = 4.07; p = 0.0063). In conclusion, determinants related to parental negative practices and to nursery policies significantly influenced children's oral health. Of particular interest was the identification of determinants considered amenable to change in connection with nursery policies towards oral health.
Related Concept Videos
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
Development of the Oral Microbiota
Tooth Anatomy
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or...
Assessment of the Mouth
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.
The Oral Microbiota
Oral Cavity
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...

