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Trihybrid Crosses02:27

Trihybrid Crosses

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Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
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A consensus map for Ug99 stem rust resistance loci in wheat.

Long-Xi Yu1, Hugues Barbier, Matthew N Rouse

  • 1Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, 14853, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|June 7, 2014
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Summary
This summary is machine-generated.

A new consensus map integrates stem rust resistance genes and markers from numerous studies. This resource aids in identifying novel resistance genes and developing wheat varieties resistant to Ug99 stem rust.

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Area of Science:

  • Plant genetics and breeding
  • Agricultural science
  • Molecular biology

Background:

  • Wheat stem rust, caused by Puccinia graminis f. sp. tritici race group Ug99, poses a significant threat to global food security.
  • Numerous studies have identified qualitative genes and quantitative trait loci (QTLs) for stem rust resistance.
  • A comprehensive resource is needed to consolidate this information for breeding efforts.

Purpose of the Study:

  • To assemble and integrate all available information on stem rust resistance loci from recent studies.
  • To construct a consensus genetic map of stem rust resistance loci in wheat.
  • To provide a valuable resource for marker-assisted selection in breeding for Ug99 resistance.

Main Methods:

  • Data from 21 recent studies on Triticum aestivum and Triticum turgidum subsp. durum were compiled.
  • The software LPmerge was utilized to construct the consensus map.
  • Diverse marker types, including SNP, DArT, GBS, and SSR, were incorporated.

Main Results:

  • A consensus wheat map was created with 1,433 markers associated with stem rust resistance.
  • 141 stem rust resistance loci conferring resistance to Ug99 were identified.
  • Several loci mapped to known Sr gene regions, while others were located in novel chromosomal regions.

Conclusions:

  • The developed consensus map serves as a crucial resource for identifying new stem rust resistance genes.
  • It facilitates the development of molecular markers for marker-assisted selection in breeding wheat for Ug99 resistance.
  • This map represents a significant advancement in the global effort to combat wheat stem rust.