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Analytical applications of circular dichroism.
N Purdie1, K A Swallows, L H Murphy
1Department of Chemistry, Oklahoma State University, Stillwater 74078-0447.
Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1989
Summary
This research introduces circular dichroism spectropolarimetry for analyzing complex mixtures, enabling drug identification and quantification without prior separation. This method offers a selective and broadly applicable approach for forensic, clinical, and pharmaceutical analysis.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Biochemistry
Background:
- Complex mixtures pose challenges for drug analysis.
- Chromatographic methods often require extensive sample preparation.
- Selective detection is crucial in forensic, clinical, and pharmaceutical settings.
Purpose of the Study:
- To develop a selective analytical detection procedure for identifying and quantifying drugs in complex mixtures.
- To avoid chromatographic separation or other work-up steps.
- To utilize circular dichroism (CD) spectropolarimetry as the detector of choice.
Main Methods:
- Circular dichroism (CD) spectropolarimetry was employed.
- The method focused on direct analysis of complex mixtures.
- Applications were explored in forensic, clinical, and pharmaceutical chemistry.
Main Results:
- Demonstrated the ability to identify and assay drugs and related substances without separation.
- Successfully analyzed enantiomeric mixtures of nicotine and cocaine.
- Analyzed D and E vitamins, cholesterol, and other steroids.
Conclusions:
- Circular dichroism spectropolarimetry provides a selective and broadly applicable method for analyzing complex mixtures.
- This technique simplifies drug analysis in various chemical disciplines.
- The developed procedure offers an efficient alternative to traditional separation-based methods.