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Human Virome

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible...

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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

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Published on: April 13, 2009

Quantitative temporal viromics: an approach to investigate host-pathogen interaction.

Michael P Weekes¹, Peter Tomasec², Edward L Huttlin³

¹Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.

|June 7, 2014

Summary

Quantitative temporal viromics (QTV) reveals how human cytomegalovirus (HCMV) manipulates host proteins during infection. This proteomic approach identified viral and cellular targets for potential therapies.

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Last Updated: Apr 28, 2026

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Area of Science:

Virology
Proteomics
Immunology

Background:

Understanding virus-host interactions is crucial for developing antiviral strategies.
Human cytomegalovirus (HCMV) serves as a model for studying viral immune evasion mechanisms.

Purpose of the Study:

To develop and apply a quantitative proteomic method for analyzing temporal changes in host and viral proteins during infection.
To gain dynamic insights into virus-host interactions and identify potential therapeutic targets.

Main Methods:

Development of quantitative temporal viromics (QTV), a multiplexed tandem-mass-tag-based mass spectrometry technique.
Application of QTV to study HCMV infection in a human cell model.

Main Results:

QTV profiled over 8,000 cellular proteins, including 1,200 cell-surface proteins, and their dynamic changes during HCMV infection.
Identified potential therapeutic targets, including natural killer and T cell ligands, and 29 cell-surface viral proteins.
Defined temporal expression profiles for over 80% of HCMV canonical genes and 14 noncanonical open reading frames.

Conclusions:

QTV is a powerful proteomic method for dissecting virus-host dynamics during infection.
The findings provide a comprehensive temporal map of HCMV infection, revealing host manipulation strategies.
QTV is broadly applicable to studying other viral infections with established in vitro models.