Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

3.8K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
3.8K
Yeast Signaling01:28

Yeast Signaling

15.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
15.6K
Transcription Elongation Factors02:35

Transcription Elongation Factors

11.1K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
11.1K
Transcription Elongation Factors02:35

Transcription Elongation Factors

3.8K
3.8K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

4.8K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.8K
Interphase00:54

Interphase

176.5K
The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
176.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The landscape of genomic and socioeconomic variables in colorectal cancer patients based on genetic ancestry.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same author

Validation of a methylation-based, tissue-free MRD assay in colorectal cancer patients from the GALAXY study.

NPJ precision oncology·2026
Same author

Identification and correction of time-series transcriptomic anomalies.

Nucleic acids research·2025
Same author

Refining the interpretation of variants of uncertain significance in hereditary cancer screening through integrated RNA sequencing.

Genetics in medicine open·2025
Same author

Generalized measures of population synchrony.

Mathematical biosciences·2024
Same author

AutoGater: a weakly supervised neural network model to gate cells in flow cytometric analyses.

Scientific reports·2024

Related Experiment Video

Updated: Apr 28, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

796

Analyzing transcription dynamics during the budding yeast cell cycle.

Adam R Leman1, Sara L Bristow, Steven B Haase

  • 1Department of Biology, Duke University, 90338, Science Drive, Durham, NC, 27708, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2014
PubMed
Summary

This study details a workflow for analyzing cell cycle transcription in budding yeast. It covers synchronizing yeast populations and analyzing RNA levels for accurate gene expression insights.

More Related Videos

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

1.7K
Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
17:01

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

Published on: July 18, 2013

12.4K

Related Experiment Videos

Last Updated: Apr 28, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

796
Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

1.7K
Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
17:01

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

Published on: July 18, 2013

12.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Understanding cell cycle-regulated transcription is crucial for deciphering fundamental biological processes.
  • Accurate measurement of global transcription requires synchronized cell populations and robust normalization techniques.

Purpose of the Study:

  • To describe a comprehensive workflow for assaying global cell cycle-regulated transcription in Saccharomyces cerevisiae.
  • To detail methods for cell synchronization, RNA isolation, and subsequent microarray analysis.
  • To outline computational approaches for comparing RNA abundance across time points and experiments.

Main Methods:

  • Synchronization of Saccharomyces cerevisiae populations using centrifugal elutriation.
  • Isolation of RNA from synchronized cell populations.
  • Microarray analysis for transcript level detection.
  • Computational methods for comparing RNA abundance data.

Main Results:

  • A complete workflow for observing RNA abundance during the cell cycle in budding yeast is established.
  • Methods allow for direct comparison of RNA abundance within and between independent experiments.

Conclusions:

  • The described workflow provides a robust framework for studying cell cycle-dependent gene expression.
  • This methodology facilitates accurate analysis of global transcription patterns throughout the cell cycle.