Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Replicative Cell Senescence

Replicative Cell Senescence

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Senescent and reactive astrocytes display distinct expression profiles and divergent functional capacities.

bioRxiv : the preprint server for biology·2026

Same author

Adenosine deaminase co-immunization reverses age-associated immunosenescence by restoring germinal center T follicular helper cell function.

bioRxiv : the preprint server for biology·2026

Same author

Trends in cryoprecipitate transfusion in the United States.

Transfusion·2026

Same author

"We have traveled into the past, which had already stretched out its shadows towards me" - What patients experience as helpful in two trauma-focused psychotherapies for PTSD related to childhood maltreatment.

Child abuse & neglect·2026

Same author

The clinical use of cryoprecipitate and fibrinogen concentrate: A scoping review.

Transfusion·2026

Same author

Interventions for common mental disorders tested in Cochrane reviews: an analysis of quality of evidence and efficacy.

World psychiatry : official journal of the World Psychiatric Association (WPA)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 28, 2026

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Detecting senescence: methods and approaches.

Elizabeth P Crowe¹, Timothy Nacarelli, Alessandro Bitto

¹Department of Pathology, Drexel University College of Medicine, 245 N. 15th Street, MS 435, Philadelphia, PA, 19102, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 8, 2014

Summary

This summary is machine-generated.

Detecting senescent cells, crucial in aging research, is challenging due to their complex nature. This study provides a stepwise, multi-technique approach for identifying these cells, particularly in aged or diseased tissues.

More Related Videos

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

Related Experiment Videos

Last Updated: Apr 28, 2026

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

Area of Science:

Gerontology and cellular biology, focusing on cellular senescence.

Background:

Cellular senescence is a key hallmark of aging and is implicated in age-related diseases.
Detecting senescent cells, especially in vivo, is difficult due to the lack of a universal biomarker and the complexity of the senescence program.

Purpose of the Study:

To outline reliable methods for detecting senescent cells.
To provide a stepwise, multi-technique strategy for senescent cell identification.

Main Methods:

Adaptation of established senescence detection methods for mesenchymal cells in culture.
Application of a stepwise protocol integrating multiple detection techniques.

Main Results:

The proposed stepwise approach facilitates senescent cell detection.
The methods are applicable to in vivo samples from aged or diseased tissues.

Conclusions:

A multi-technique, stepwise approach enhances the accuracy of senescent cell detection.
This strategy addresses the challenges of identifying senescent cells in complex biological contexts.