Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes01:29

Replication in Eukaryotes

15.3K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.3K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.1K
Replication in Prokaryotes01:32

Replication in Prokaryotes

20.4K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
20.4K
Replication in Prokaryotes02:35

Replication in Prokaryotes

48.7K
48.7K
Replication in Prokaryotes02:35

Replication in Prokaryotes

86.5K
Overview
86.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automatic detection of early gastric cancer in endoscopy based on Mask region-based convolutional neural networks (Mask R-CNN)(with video).

Frontiers in oncology·2022
Same author

Development and validation of a pyradiomics signature to predict initial treatment response and prognosis during transarterial chemoembolization in hepatocellular carcinoma.

Frontiers in oncology·2022
Same author

Natural Course for Retinal Detachment in Morning Glory Disc Anomaly Based on a Grading System.

Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)·2022
Same author

Heterogeneity of T cells and macrophages in chlorine-induced acute lung injury in mice using single-cell RNA sequencing.

Inhalation toxicology·2022
Same author

Predictive Value of the Hepatic Immune Predictive Index for Patients with Primary Liver Cancer Treated with Immune Checkpoint Inhibitors.

Digestive diseases (Basel, Switzerland)·2022
Same author

Secure OFDM-PON using three-dimensional selective probabilistic shaping and chaos.

Optics express·2022

Related Experiment Video

Updated: Apr 28, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

796

Analysis of replication timing using synchronized budding yeast cultures.

Jie Peng1, M K Raghuraman, Wenyi Feng

  • 1Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, 4287 Weiskotten Hall, 750 East Adams Street, Syracuse, NY, 13210, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2014
PubMed
Summary
This summary is machine-generated.

Understanding eukaryotic DNA replication requires advanced methods to analyze its temporal program. New techniques offer insights into DNA synthesis and genome maintenance across diverse organisms.

More Related Videos

Measuring Replicative Life Span in the Budding Yeast
12:41

Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

20.4K
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

1.7K

Related Experiment Videos

Last Updated: Apr 28, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

796
Measuring Replicative Life Span in the Budding Yeast
12:41

Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

20.4K
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

1.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic DNA replication is crucial for cell division, displaying both high accuracy and flexibility.
  • The existence and significance of a population-level replication temporal program are currently debated.
  • Advancements in methodologies allow for detailed analysis of DNA replication dynamics.

Purpose of the Study:

  • To explore the role and regulation of DNA replication timing.
  • To discuss the interpretation challenges and applications of current replication analysis methods.
  • To highlight the necessity of applying diverse methods across various genetic backgrounds and organisms.

Main Methods:

  • Global analysis of replication dynamics in model organisms.
  • Single-molecule level analysis of DNA replication.
  • Utilizing various experimental techniques to study replication processes.

Main Results:

  • Current methods provide unique perspectives on DNA replication.
  • Each method presents distinct challenges in interpreting experimental data.
  • Replication dynamics can be studied at both population and single-molecule levels.

Conclusions:

  • Further application of diverse analytical methods is essential for a comprehensive understanding of DNA replication.
  • Investigating replication in different genetic contexts will enhance knowledge of genome maintenance.
  • Improved understanding of DNA replication regulation impacts overall genome stability.