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Recombinant human tyrosyl-tRNA synthetase, a novel thrombopoietic agent
Zhan Ling1, Zhang Yanling1, Feng Zhe1
1The Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, China; The Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, China.
Abstract:
Chemo- and radiotherapy induced thrombocytopenia present significant limitations for tumor therapy. In this study, we examined the therapeutic effect of recombinant human tyrosyl-tRNA synthetase (rhTyrRS) against development of thrombocytopenia in cyclophosphamide (CTX) treated mice. Our data indicate that intraperitoneal administration of at least 10 μg/day rhTyrRS for 7 days significantly prevents the decrease in platelets 8 days after CTX injection. The data further demonstrate that rhTyrRS promotes migration and aggregation of megakaryocytes to the bone marrow niche. Real-time PCR and flow cytometry reveal that VCAM-1 is particularly important for the adhesion. Together, these data suggest that rhTyrRS might be a useful therapeutic agent for chemotherapy-induced thrombocytopenia.
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