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Published on: August 10, 2018
Effects of human amniotic mesenchymal stromal cells on rabbit T-cell responses in a xenolymphocyte reaction assay
Yifeng Xue1, Zongning Miao, Hongli Sun
1Nanjing Medical University, Affiliated Wuxi Peoples Hospital, Department of Clinical Laboratory, Wuxi, Jiangsu Province, China.
Objectives:
To evaluate the immunomodulatory properties of human amniotic mesenchymal stromal cells.
Materials And Methods:
Human amniotic mesenchymal stromal cells were isolated, characterized by flow cytometry, cultured in vitro, and evaluated in allogeneic and xenogeneic mixed lymphocyte reactions. The proliferation of T cells and the expression of interleukin 2 and interferon gamma by T cells were evaluated in the presence of human amniotic mesenchymal stromal cells.
Results:
Human amniotic mesenchymal stromal cells were successfully isolated from human amniotic membranes and had well-defined human mesenchymal stem cell markers (CD90, CD73, CD105, and CD166). The human amniotic mesenchymal stromal cells inhibited the proliferation of human and rabbit T cells and the secretion of interleukin-2 and interferon gamma by human T cells.
Conclusions:
Human amniotic mesenchymal stromal cells may be useful for cell therapy and tissue engineering because of availability, phenotypic plasticity, and immunomodulatory properties.
