Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analytical oxidation of reserpine.

S Ebel1, W Mück, F J Placke

  • 1Department of Pharmacy, Bayerische Julius Maximilians Universität, Würzburg, FRG.

Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hepatic chemosaturation with melphalan in patients with primary or secondary liver tumors with or without extrahepatic tumor manifestation.

ESMO gastrointestinal oncology·2026
Same author

High Plaque Levels after Thorough Toothbrushing: What Impedes Efficacy?

JDR clinical and translational research·2019
Same author

First acute haemodynamic study of soluble guanylate cyclase stimulator riociguat in pulmonary hypertension.

The European respiratory journal·2009
Same author

Analysis of atropine, its degradation products and related substances of natural origin by means of reversed-phase high-performance liquid chromatography.

Journal of chromatography. A·2004
Same author

Clinical-pharmacological strategies to assess drug interaction potential during drug development.

Drug safety·2001
Same author

Pharmacokinetics of cerivastatin in renal impairment are predicted by low serum albumin concentration rather than by low creatinine clearance.

Journal of clinical pharmacology·2001

Electrochemical oxidation of reserpine yields 3,4-dehydroreserpine, not the expected 10-hydroxy-reserpine. This finding was confirmed using HPLC and impacts reserpine analysis methods.

Area of Science:

  • Analytical Chemistry
  • Organic Chemistry
  • Electrochemistry

Background:

  • Reserpine analysis often involves oxidation, with previous studies reporting specific products.
  • Understanding oxidation pathways is crucial for accurate drug quantification and quality control.

Purpose of the Study:

  • To investigate the electrochemical oxidation products of reserpine in an acidic medium.
  • To compare electrochemical oxidation with chemical oxidation using sodium nitrite.
  • To validate spectrophotometric assay conditions using advanced analytical techniques.

Main Methods:

  • Electrochemical (anodic) oxidation of reserpine in acidic medium.
  • Chemical oxidation using sodium nitrite.
  • High-Performance Liquid Chromatography (HPLC) with a photodiode-array detector.

Related Experiment Videos

  • Spectrophotometric analysis.

    • Main Results:

    • Electrochemical oxidation predominantly formed 3,4-dehydroreserpine, contrary to the expected 10-hydroxy-reserpine.
    • Both electrochemical and chemical oxidation with sodium nitrite quantitatively yielded the same primary product.
    • Minimal by-products were observed when the reaction was protected from light.
    • HPLC analysis confirmed the validity of the European Pharmacopoeia's spectrophotometric assay conditions.

    Conclusions:

    • The primary product of reserpine's electrochemical oxidation in acid is 3,4-dehydroreserpine.
    • Electrochemical and sodium nitrite oxidation pathways converge to the same major product.
    • The study validates existing spectrophotometric assay methods for reserpine while clarifying its oxidation chemistry.