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Related Experiment Video

Updated: Apr 28, 2026

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

Etchable plasmonic nanoparticle probes to image and quantify cellular internalization.

Gary B Braun¹, Tomas Friman¹, Hong-Bo Pang²

¹1] Cancer Research Center, Sanford-Burnham Medical Research Institute, La Jolla, California 92037, USA [2] Center for Nanomedicine and Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, USA.

Nature Materials

|June 9, 2014

Summary

This summary is machine-generated.

Researchers developed a novel method to distinguish internalized nanoparticles from surface-bound ones. This technique uses silver nanoparticles (AgNPs) that can be selectively removed from cell surfaces, enabling clearer imaging and quantification of intracellular delivery.

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Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

Area of Science:

Biomedical Engineering
Nanotechnology
Cell Biology

Background:

Nanoparticles are widely explored for cellular labeling and drug delivery.
Current fluorescent imaging methods struggle to differentiate cell surface-bound from internalized nanoparticles.

Purpose of the Study:

To develop a technique for unequivocally distinguishing cell surface-bound from internalized nanoparticles.
To enable accurate quantification and imaging of intracellular nanoparticle localization.

Main Methods:

A novel silver nanoparticle (AgNP) etching technique using a hexacyanoferrate-thiosulphate redox solution.
Development of multicoloured, dye-labeled, peptide-functionalized AgNPs.
In vitro and in vivo testing in mice, including toxicity and tumor uptake studies.

Main Results:

The AgNP etching method effectively removes external nanoparticles, leaving only the internalized pool.
Developed nanoprobes are bright, photostable, and show minimal toxicity in vivo.
Demonstrated successful tumor uptake of the nanoprobes in mice.

Conclusions:

The developed etching technique provides a reliable way to assess nanoparticle internalization.
Multicoloured plasmonic nanoprobes offer a promising tool for advanced cellular imaging and targeted delivery applications.