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A single nucleotide polymorphism in PIK3CA gene is inversely associated with P53 protein expression in breast cancer

Bo Pang1, Shi-Peng Sun, Lei Gao

  • 1Clinical Laboratory, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beixian Ge 5#, XiCheng District, Beijing, 100053, China.

Medical Oncology (Northwood, London, England)
|June 9, 2014
PubMed

Insights

Single nucleotide polymorphism (SNP) rs17849071’s GT+GG genotype is inversely associated with P53 protein accumulation in Chinese breast cancer (BCa) patients. This finding may offer new insights into BCa pathogenesis and treatment strategies.

Area of Science:

  • Genetics
  • Oncology
  • Molecular Biology

Background:

  • Single nucleotide polymorphism (SNP) rs17849071 has been linked to PIK3CA amplification in thyroid cancer.
  • The precise role of SNP rs17849071 in other cancers, like breast cancer (BCa), remains largely uncharacterized.

Purpose of the Study:

  • To investigate the association between SNP rs17849071 and P53 expression status in Chinese breast cancer patients.
  • To explore the relationship between SNP rs17849071 and other clinicopathological characteristics of breast cancer.

Main Methods:

  • Polymerase Chain Reaction (PCR) and sequencing were employed to genotype SNP rs17849071.
  • P53 protein expression levels were assessed in 62 Chinese breast cancer samples.

Main Results:

  • P53 protein accumulation showed significant associations with HER2 overexpression (P = 0.013) and Ki-67 expression (P = 0.007).
  • A significant inverse relationship was observed between P53 protein expression and the rs17849071 GT+GG genotype (P = 0.044).
  • SNP rs17849071 was not found to be related to estrogen receptor, progestin receptor, or HER2 status, nor did it differ across breast cancer intrinsic subtypes.

Conclusions:

  • The rs17849071 GT+GG genotype is inversely associated with P53 protein accumulation in breast cancer.
  • Further large-scale studies are warranted to elucidate the complex interplay between rs17849071 polymorphisms, P53 status, and PIK3CA amplification in breast cancer.

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