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Analysis of the released nuclear cytokine HMGB1 in human serum
Haichao Wang1, Lin Zhao, Jianhua Li
1Laboratory of Emergency Medicine, The Feinstein Institute for Medical Research, North Shore-LIJ Health System, 350 Community Drive, 11030, Manhasset, NY, USA, hwang@nshs.edu.
Methods in Molecular Biology (Clifton, N.J.)
|June 9, 2014
Abstract:
A ubiquitous nuclear protein, the high-mobility group box 1 (HMGB1), is secreted by activated macrophages/monocytes and leaked passively from injured cells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.
