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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

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ELISA-based assay for IP-10 detection from filter paper samples.

Camilla Heldbjerg Drabe¹, Thomas Blauenfeldt, Morten Ruhwald

¹Department of Pulmonary and Infectious Diseases, Copenhagen University Hospital of North Zealand, Hillerød, Denmark, camilla.h.drabe@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|June 9, 2014

Summary

This study presents a new ELISA method for detecting Interferon gamma-induced protein 10 (IP-10) in dried blood spots. This advance aids in diagnosing inflammatory diseases and infections like tuberculosis.

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Area of Science:

Biochemistry
Immunology
Molecular Biology

Background:

Interferon gamma-induced protein 10 (IP-10) is a pro-inflammatory chemokine.
Elevated IP-10 levels are linked to various inflammatory conditions, including infections and cancer.
IP-10 serves as a biomarker for disease severity and aids in diagnosing infections like tuberculosis and cytomegalovirus.

Purpose of the Study:

To develop and validate an Enzyme-Linked Immunosorbent Assay (ELISA) for quantifying IP-10.
To enable IP-10 detection in convenient dried blood spot (DBS) and plasma samples.
To facilitate improved diagnostics for inflammatory and infectious diseases.

Main Methods:

Development of a sensitive ELISA protocol.
Utilization of dried blood spot (DBS) and plasma samples.
Validation of the assay for IP-10 detection.

Main Results:

Successful establishment of an ELISA method for IP-10 detection.
Demonstration of IP-10 detectability in DBS and plasma samples.
Potential for this method in clinical diagnostics.

Conclusions:

The developed ELISA offers a reliable method for IP-10 measurement.
This technique supports the use of IP-10 as a biomarker in resource-limited settings using DBS.
The assay can aid in the immunodiagnostics of various inflammatory conditions and infections.