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Updated: Apr 28, 2026
An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
Published on: June 23, 2017
Cytokine-induced neutrophil chemotaxis assay
Dennis Davidson1, Hardik Patel
1Division of Neonatal-Perinatal Medicine, Stony Brook Long Island Children's Hospital, Stony Brook University, Health Science Center Building, 11th Floor, Room 060, Stony Brook, NY, 11794, USA, Dennis.Davidson@stonybrookmedicine.edu.
Abstract:
Chemotaxis is directed migration of a cell type to a distant chemoattractant. When this chemoattractant is a cytokine, the term chemokine is often used. Chemotaxis by neutrophils, specifically polymorphonuclear leukocytes (PMNs), plays a critical role in the innate immune response. On an equimolar basis, interleukin-8 (IL-8) is one of the most potent PMN chemokines known. This chapter describes an in vitro chemotaxis technique using PMNs and IL-8 which can serve the investigator as an established model from which new studies can be developed.
