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Functional toll-like receptor 3 expressed by oral squamous cell carcinoma induced cell apoptosis and decreased

Zhifeng He1, Xiaofeng Huang1, Yanhong Ni1

  • 1Central Laboratory of Stomatology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China.

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
|June 9, 2014
PubMed
Abstract

Insights

Toll-like receptor 3 (TLR3) is present in oral squamous cell carcinoma (OSCC). Activating TLR3 with Poly(I:C) reduces OSCC cell viability and migration, suggesting TLR3 as a potential immunotherapy target.

Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent malignancy.
  • The role of pattern recognition receptors, such as toll-like receptors (TLRs), in OSCC is not fully understood.

Purpose of the Study:

  • To investigate the expression and functional role of toll-like receptor 3 (TLR3) in oral squamous cell carcinoma (OSCC).

Main Methods:

  • TLR3 expression was assessed in primary OSCC tissues and cell lines (SCC4, CAL27).
  • OSCC cell lines were treated with polyinosinic-polycytidylic acid [Poly(I:C)] to activate TLR3.
  • Changes in cytokine expression, cell viability, apoptosis, and migration were analyzed.

Main Results:

  • TLR3 was detected in both OSCC tissue and cell lines.
  • Poly(I:C) stimulation upregulated cytokine expression in OSCC cells.
  • Activation of TLR3 led to decreased cell viability (via suppressed proliferation and induced apoptosis) and reduced cell migration.
  • Poly(I:C)-TLR3-induced apoptosis was dependent on caspase-3.

Conclusions:

  • TLR3 is expressed in OSCC and its activation influences cancer cell behavior.
  • TLR3 may play a role in OSCC development.
  • TLR3 represents a potential therapeutic target for OSCC immunotherapy.

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