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Related Concept Videos

Tight Junctions01:29

Tight Junctions

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Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
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Cytoskeletal Linker Proteins - Plakins01:09

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Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
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Cadherins in Tissue Organization01:19

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The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
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Related Experiment Video

Updated: Apr 28, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
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Dermatopontin is a novel regulator of the CdCl2-induced decrease in claudin-11 expression.

Qiaozhen Yang1, Jie Hao2, Maoxin Chen1

  • 1Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|June 10, 2014
PubMed
Summary

Cadmium chloride exposure increases dermatopontin (DPT) in Sertoli cells, damaging the blood-testis barrier (BTB). DPT silencing partially reverses this damage, implicating DPT in cadmium

Keywords:
Blood-testis barrierCdCl(2)Claudin-11Dermatopontin

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Area of Science:

  • Reproductive toxicology
  • Environmental health
  • Cell biology

Background:

  • Cadmium (Cd) is an environmental heavy metal toxicant.
  • Cd exposure can impair male reproductive function by damaging the blood-testis barrier (BTB).
  • The specific mechanisms underlying Cd-induced BTB damage are not fully understood.

Purpose of the Study:

  • To investigate the role of dermatopontin (DPT) in cadmium chloride (CdCl2)-induced BTB damage.
  • To elucidate the molecular mechanisms by which CdCl2 affects BTB integrity.
  • To identify potential therapeutic targets for mitigating Cd-induced reproductive toxicity.

Main Methods:

  • In vivo and in vitro studies using Sertoli cells and animal models.
  • Assessment of DPT and claudin-11 expression levels following CdCl2 treatment.
  • Establishment of a DPT-silenced Sertoli cell model.
  • Western blot analysis to investigate signaling pathway involvement (p38).

Main Results:

  • Dermatopontin (DPT) expression is significantly upregulated in Sertoli cells upon CdCl2 exposure, both in vivo and in vitro.
  • CdCl2 treatment leads to a concurrent decrease in claudin-11 expression, a key BTB component.
  • Silencing DPT partially mitigates the CdCl2-induced reduction in claudin-11 expression.
  • The p38 signaling pathway is implicated in CdCl2-induced DPT modulation.

Conclusions:

  • Dermatopontin (DPT) emerges as a novel mediator in cadmium chloride (CdCl2)-induced testicular toxicity.
  • DPT plays a significant role in regulating claudin-11 expression and maintaining blood-testis barrier (BTB) integrity.
  • These findings provide new insights into the mechanisms of reproductive toxicity caused by environmental cadmium exposure.