Related Experiment Video
Updated: Apr 28, 2026
NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
Published on: December 30, 2025
Fluorescent hydrogen peroxide sensor based on cupric oxide nanoparticles and its application for glucose and
Ai-Ling Hu1, Yin-Huan Liu2, Hao-Hua Deng1
1Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
Abstract:
A novel fluorescent hydrogen peroxide sensor was developed based on the peroxidase-like activity of cupric oxide nanoparticles. Cupric oxide nanoparticles effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals. Then terephthalic acid was oxidized by hydroxyl radical to form a highly fluorescent product. The linear range of hydrogen peroxide estimated to be 5.0 × 10(-6)-2.0 × 10(-4)M with a detection limit of 3.4 × 10(-7)M. Moreover, this detection system enabled the sensing of analytes which can enzymatically generate hydrogen peroxide. By coupling the oxidation of glucose or L-lactate catalyzed by their corresponding oxidase enzymes with terephthalic acid oxidation catalyzed by cupric oxide nanoparticles, sensitive assays of glucose and l-lactate with detection limits of 1.0 × 10(-6) and 4.5 × 10(-8)M were realized. The successful applications of this approach in human serum samples have also been demonstrated.
