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Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome
Karen L Nielsen1, Paul A Godfrey2, Marc Stegger3
1Department of Clinical Microbiology, Hvidovre Hospital, 2640 Hvidovre, Denmark; Department of Microbiology and Infection Control, Statens Serum Institut, 2300 Copenhagen S, Denmark.
Journal of Microbiological Methods
|June 10, 2014
Abstract:
Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.

