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[In vitro sensitivity of 3 strains of Leptospira interrogans to 3 different antibiotics]
R Benzi Cipelli1, G Gorini, S Caligaris
1Istituto di Clinica delle Malattie Infettive, Università di Pavia, IRCCS Ospedale Policlinico San Matteo.
Abstract:
Leptospirosis, anthropo-zoonosis ubiquitously widespread, is a social and economic problem still to be solved. The experimental and therapeutic employment of many antibiotics has largely been tested "in vitro" and "in vivo". In the following research we tried to evaluate, by experimental "in vitro" method, the sensitivity difference of three serovar strains of Leptospira interrogans to two macrolides, Erythromycin and Josamycin, compared with Penicillin. From standard cultures, previously treated with serial dilution of these antibiotics, the MIC and MSC, as quantitative parameters, have been stated. For the qualitative evaluation of the damages induced at ultrastructural level by the drug activity. Electron Microscopy investigations were performed on specimens from cultures treated for 6 hr with twice and tenfold the MSC. The present research confirms the good sterilizing efficaciousness "in vitro" of the tested macrolides (MSC less than 1 mcg/ml) and their different activity pathway.
Insights
This study found that macrolide antibiotics Erythromycin and Josamycin are effective in vitro against Leptospira interrogans. Both macrolides demonstrated sterilizing efficacy, with minimal inhibitory concentrations below 1 mcg/ml.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Leptospirosis is a widespread zoonotic disease with significant social and economic impact.
- Antibiotic efficacy against Leptospira interrogans requires further investigation for optimal treatment strategies.
Purpose of the Study:
- To evaluate the in vitro sensitivity of Leptospira interrogans serovars to Erythromycin and Josamycin compared to Penicillin.
- To assess the ultrastructural damage induced by these antibiotics on Leptospira interrogans.
Main Methods:
- In vitro antibiotic sensitivity testing using serial dilutions to determine Minimum Inhibitory Concentration (MIC) and Minimum Sterilizing Concentration (MSC).
- Electron Microscopy was employed to examine ultrastructural changes in Leptospira interrogans after antibiotic exposure.
Main Results:
- Both Erythromycin and Josamycin exhibited significant in vitro sterilizing efficacy against Leptospira interrogans strains.
- The Minimum Sterilizing Concentrations (MSC) for the macrolides were found to be less than 1 mcg/ml.
- Distinct pathways of antibiotic activity were observed for the macrolides.
Conclusions:
- Macrolides, specifically Erythromycin and Josamycin, are potent in vitro agents against Leptospira interrogans.
- These macrolides offer a promising alternative or adjunct therapy for leptospirosis, warranting further in vivo studies.