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Updated: Apr 28, 2026
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Integrated microRNA-mRNA analysis of coronary artery disease
Fei Chen1, Xin Zhao, Juan Peng
1Department of Cardiology, Fifth Hospital of Fudan University, Shanghai, 200240, China.
Insights
This study identifies key microRNAs (miRNAs) and their target genes involved in coronary artery disease (CAD) pathogenesis. These differentially expressed miRNAs may offer new therapeutic targets for treating CAD.
Area of Science:
- Cardiovascular Biology
- Molecular Genetics
- Bioinformatics
Background:
- Coronary artery disease (CAD) presents a high mortality rate, yet its pathogenesis remains incompletely understood.
- Identifying molecular mechanisms is crucial for developing effective therapeutic strategies for CAD.
Purpose of the Study:
- To investigate the molecular mechanisms underlying CAD pathogenesis.
- To identify potential molecular targets for CAD intervention.
Main Methods:
- Downloaded and analyzed platelet microRNA (miRNA) and blood messenger RNA (mRNA) expression profiles from Gene Expression Omnibus (GSE28858, GSE42148).
- Identified differentially expressed genes (DEGs) and miRNAs using microarray analysis.
- Constructed a miRNA-target gene regulatory network using the miRecords database and Spearman correlation analysis.
Main Results:
- Screened six up-regulated (miR-340, miR-545, miR-451, miR454-5p, miR-624, miR-585) and four down-regulated (miR-199a, miR-17-3p, miR-154, miR-339) miRNAs in CAD patients.
- Predicted 295 target genes for specific miRNAs, with 7 identified as DEGs.
- Found strong positive correlations between miR-545 and TFEC (ρ = 0.808), and miR-585 and SPOCK1 (ρ = 0.875) in CAD samples.
Conclusions:
- Differentially expressed miRNAs play a role in CAD pathogenesis by regulating target genes.
- The identified miRNA-target gene interactions, such as miR-545-TFEC and miR-585-SPOCK1, represent potential therapeutic targets for CAD.
Abstract:
Although patients with coronary artery disease (CAD) have a high mortality rate, the pathogenesis of CAD is still poorly understood. The purpose of this study was to explore the underlying molecular mechanisms and potential target molecules for CAD. The platelet miRNA (GSE28858) and blood mRNA (GSE42148) expression profiles of patients with CAD and healthy controls were downloaded from Gene Expression Omnibus. Differentially expressed miRNAs and genes (DEGs) were identified by significant analysis of microarray algorithm after data preprocessing. Furthermore, the miRNA-target gene regulatory network was constructed based on miRecords database. The spearman correlation coefficients (ρ) between miRNAs and their target genes were calculated. Six up- (miR-340, miR-545, miR-451, miR454-5p, miR-624 and miR-585) and four down-regulated (miR-199a, miR-17-3p, miR-154 and miR-339) miRNAs were screened. Total 295 target genes of miR-545, miR-451, miR-585 and miR-154 were predicted. Among these 295 target genes, 7 genes were DEGs. Further analysis showed miR-545-TFEC and miR-585-SPOCK1 were highly positively correlated (ρ = 0.808091264; ρ = 0.874680776) in CAD samples. Therefore, differentially expressed miRNAs might participate in the pathogenesis of CAD by regulating their target genes.
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