Establishment of Panax notoginseng tissue culture

Calli were induced from Panax notoginseng roots, tubers, stems, petioles, leaves, and flower buds, and preliminary identification of medicinal compounds in the callus was carried out by TLC, using subcultured sixth-generation calli. The results demonstrated that callus was capable of synthesizing saponins, including ginsenosides Rb1, Rg1, and Rh1. The saponin content (5.33%, D.W.) in the callus was higher than that in intact plants (4.25%, D.W.). Callus also produced a larger amount of sapogenins than intact plants. Callus growth rate was 54.0 mg D.W./liter/day. Callus induced from stems was superior in terms of growth, saponin and sapogenin contents, and saponin composition. MS medium proved optimal for growth of callus induced from stems. The optimum pH was about 5.8 and the optimum temperature about 26 degrees C. Light had little stimulatory effect on callus growth.