Purification of ovine prolactin by affinity chromatography with monoclonal antibodies

Five monoclonal antibodies (McAbs) against ovine prolactin (oPRL) were obtained by the lymphocyte hybridoma technique. One low-affinity McAb with (2D2) was purified by hydroxylapatite chromatography. The 2D2 was immobilized on Sepharose 4B with epichlorohydrin to produce an immunosorbent column, which was used to purify oPRL from a crude preparation by a single-step procedure.