Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein extraction with reverse micelles.

H Q Shi1, L Xia, R Guo

  • 1Department of Chemical Engineering, Tsinghua University, Beijing.

Chinese Journal of Biotechnology
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment outcome of extracorporeal membrane oxygenation in critically ill patients with pulmonary TB.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2026
Same author

Precise Measurement of the Chromoelectric Dipole Moment of the Charm Quark.

Physical review letters·2026
Same author

Precise Measurement of Matter-Antimatter Asymmetry with Entangled Hyperon-Antihyperon Pairs.

Physical review letters·2026
Same author

Observation of Λ[over ¯]p→K^{+}π^{+}π^{-}π^{0} and Λ[over ¯]p→K^{+}π^{+}π^{-}2π^{0}.

Physical review letters·2026
Same author

First Measurement of the D_{s}^{+}→K^{0}μ^{+}ν_{μ} Decay.

Physical review letters·2026
Same author

Observation of the Electromagnetic Radiative Decays of the Λ(1520) and Λ(1690) to γΣ^{0}.

Physical review letters·2026
Same journal

Detection and nucleotide analysis of RNA5 of beet necrotic yellow vein virus isolated in China.

Chinese journal of biotechnology·2000
Same journal

Continuous ethanol fermentation using the fusion strain SPSC (I) flocs: floc size distribution, cell growth, and ethanol fermentation kinetics.

Chinese journal of biotechnology·2000
Same journal

Study on rt-PA production by recombinant CHO cells immobilized with a porous microcarrier.

Chinese journal of biotechnology·2000
Same journal

Optimization of conditions for safflower cell culture and accumulation of cellicolous product tocopherols.

Chinese journal of biotechnology·2000
Same journal

Gene fusion and expression of heat-labile and heat-stable enterotoxins of enterotoxigenic Escherichia coli.

Chinese journal of biotechnology·2000
Same journal

Expression of rainbow trout growth hormone cDNA in Saccharomyces cerevisiae.

Chinese journal of biotechnology·2000
See all related articles

Reverse micelle liquid-liquid extraction offers efficient protein separation. This study optimized conditions for extracting lysozyme, trypsin, and pepsin, finding low-molecular-mass proteins were extracted most effectively.

Area of Science:

  • Biochemistry
  • Separation Science
  • Chemical Engineering

Background:

  • Liquid-liquid extraction is a key technique in protein purification.
  • Reverse micelle systems offer a novel approach for biomolecule separation.
  • Optimizing extraction parameters is crucial for efficient protein recovery.

Purpose of the Study:

  • To investigate the efficiency of an AOT/isooctane reverse micelle system for protein extraction.
  • To determine the impact of pH, ionic concentration, cation type, and protein molecular mass on extraction performance.
  • To evaluate the extraction of specific proteins: lysozyme, trypsin, and pepsin.

Main Methods:

  • Utilized a reverse micelle system composed of AOT (bis(2-ethylhexyl) sulfosuccinate) in isooctane.

Related Experiment Videos

  • Systematically varied parameters including pH, ionic concentration, and cation type.
  • Assessed extraction efficiency based on protein molecular mass for lysozyme, trypsin, and pepsin.

    • Main Results:

    • The AOT/isooctane reverse micelle system demonstrated high efficiency in protein extraction.
    • Extraction performance was significantly influenced by solution pH and ionic conditions.
    • Low-molecular-mass proteins, such as lysozyme, trypsin, and pepsin, were extracted with notable efficiency.

    Conclusions:

    • Reverse micelle-based liquid-liquid extraction is a viable and effective method for protein separation.
    • Optimized conditions can enhance the recovery of target proteins.
    • The system shows particular promise for the extraction of smaller proteins.