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Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Published on: August 5, 2021
The annulate lamellae--from obscurity to spotlight
1University of Iowa, Iowa City 52242.
Electron Microscopy Reviews
|January 1, 1989
Summary
Annulate lamellae, found in various cells including sex and cancer cells, are reviewed. Their structure, origin, and function remain enigmatic, requiring further investigation with modern cell biology techniques.
Area of Science:
- Cell Biology
- Ultrastructure Research
Background:
- Annulate lamellae are a widespread organelle found in diverse cell types, including gametes, tumor cells, somatic cells, and plant cells.
- Their presence in metabolically active and differentiating cells suggests potential functional significance.
Purpose of the Study:
- To provide a comprehensive survey of annulate lamellae, focusing on their distribution, ultrastructure, and biogenesis.
- To explore the relationship of annulate lamellae with other cellular structures, particularly the nuclear envelope and endoplasmic reticulum.
- To summarize current knowledge and hypotheses regarding annulate lamellae function.
Main Methods:
- Electron microscopy and associated experimental techniques were emphasized for studying annulate lamellae.
- Review of experimental manipulations that alter annulate lamellae abundance.
- Compilation of existing literature and bibliographical data.
Main Results:
- Electron microscopy has elucidated the distribution, ultrastructure, and morphogenesis of annulate lamellae.
- Experimental studies demonstrate that annulate lamellae quantity can be modulated.
- The precise function of annulate lamellae remains largely undetermined.
Conclusions:
- Annulate lamellae are a ubiquitous organelle with poorly understood functions.
- Further research utilizing advanced cell and molecular biology techniques is needed to elucidate their role.
- This review aims to stimulate interest and guide future investigations into annulate lamellae.
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