Related Experiment Video
Updated: Apr 28, 2026
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
Published on: November 5, 2019
6.2K
Neoplastic plasma cell aberrant antigen expression patterns and their association with genetic abnormalities
Mohamed E Salama1, Shouying Du, Olga Efimova
1ARUP Institute for Experimental Pathology.
Leukemia & Lymphoma
|June 11, 2014
Summary
Aberrant antigen expression in multiple myeloma (MM) correlates with specific genetic changes. Understanding these patterns offers potential diagnostic and prognostic value for MM patients.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Aberrant antigen expression in multiple myeloma (MM) and its link to cytogenetic aberrations remain poorly understood.
- Phenotypic variations in MM plasma cells (PCs) may reflect underlying genetic heterogeneity.
Purpose of the Study:
- To investigate the association between antigen expression patterns and cytogenetic aberrations in MM.
- To correlate flow cytometry and fluorescence in situ hybridization (FISH) findings with gene expression profiles.
Main Methods:
- Analysis of 167 bone marrow specimens from MM patients using flow cytometry and FISH.
- Gene expression profiling of CD56, CD117, CD52, and CD20 mRNA in plasma cells.
- Evaluation of correlations between antigen expression, cytogenetic findings, and molecular subgroups (c-MAF, FGFR3).
Main Results:
- Higher CD56 and CD117 expression correlated with hyperdiploidy.
- High CD52 mRNA expression was linked to c-MAF and FGFR3 subgroups.
- CKS1B amplification showed a positive correlation with CD52 and a negative correlation with CD20.
- Distinct Kit and NCAM mRNA expression patterns were observed in c-MAF and FGFR3 subgroups.
Conclusions:
- Phenotypic differences in MM are associated with specific genetic subgroups.
- These associations hold potential diagnostic and prognostic significance for multiple myeloma.
